Rippe, Karsten and Schrader, Anna and Riede, Philipp and Strohner, Ralf and Lehmann, Elisabeth and Laengst, Gernot (2007) DNA sequence- and conformation-directed positioning of nucleosomes by chromatin-remodeling complexes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104 (40). pp. 15635-15640. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
Chromatin-remodeling complexes can translocate nucleosomes along the DNA in an ATP-coupled reaction. This process is an important regulator of all DNA-dependent processes because it determines whether certain DNA sequences are found in regions between nucleosomes with increased accessibility for other factors or wrapped around the histone octamer complex. In a comparison of seven different chromatin-remodeling machines (ACF, ISWI, Snf2H, Chd1, Mi-2, Brg1, and NURF), it is demonstrated that these complexes can read out DNA sequence features to establish specific nucleosome-positioning patterns. For one of the remodelers, ACF, we identified a 40-bp DNA sequence element that directs nucleosome positioning. Furthermore, we show that nucleosome positioning by the remodelers ACF and Chd1 is determined by a reduced affinity to the end product of the translocation reaction. The results suggest that the linkage of differential remodeling activities with the intrinsic binding preferences of nucleosomes can result in establishing distinct chromatin structures that depend on the DNA sequence and define the DNA accessibility for other protein factors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | YEAST ALPHA-2 REPRESSOR; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL REGULATION; GENE-TRANSCRIPTION; HISTONE OCTAMER; SWI-SNF; ISWI; DROSOPHILA; EXPRESSION; PROMOTER; ACF; nucleosome remodeling; nucleosome positioning |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 10:38 |
| Last Modified: | 01 Dec 2020 10:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32085 |
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