Denzinger, Stefan and Stoehr, Robert and Schwarz, Stephan and Eichenseher, Nicole and Brockhoff, Gero and Obermann, Ellen C. and Knuechel, Ruth and Blaszyk, Hagen and Hartmann, Arndt and Wild, Peter J. (2007) Low level STK15 amplification in histologically benign urothelium of patients with bladder cancer adversely predicts patient outcome following cystectomy. INTERNATIONAL JOURNAL OF ONCOLOGY, 31 (4). pp. 793-802. ISSN 1019-6439,
Full text not available from this repository. (Request a copy)Abstract
The aim of this study was to investigate STK15 amplification in histologically benign urothelium and invasive tumor tissue of urothelial bladder cancer patients in relation to clinicopathologic and molecular characteristics, and to analyze a hypothesized association between the STK15 single nucleotide polymorphism at site T91A (Phe31Ile) and STK15 gene amplification. A tissue microarray (TMA) was constructed and contained formalin-fixed paraffin-embedded tumor tissue and matching histologically benign urothelium of 44 patients who underwent cystectomy for invasive urothelial carcinoma. Expression of TP53, CK20 and MIB1 was evaluated by immunohistochemistry. UroVysion and STK15 fluorescence in situ hybridization (FISH) analysis was performed for sensitive detection of polysomy, relative p16 deletion and STK15 amplification, respectively. Genotypes of STK15 at the T91A (Phe31Ile) site were analyzed by PCR-RFLP assay. Low level STK15 amplification was found in 2 of 36 analyzable histologically benign urothelium specimens (5.6%) and in 64% (28/44) of urothelial bladder cancers, whereas 36% (16/44) of cancer lesions showed high level of STK15 amplification. In histologically benign urothelium of bladder cancer patients, low level STK15 amplification was associated with shorter recurrence-free and tumor-specific survival. There was no correlation between allelic variants and high/low level of STK15 gene amplification. Applying STK15 FISH to benign urothelium of bladder cancer patients may help to identify patients at increased risk for adverse clinical outcome. A large randomized prospective study comparing early versus delayed cystectomy in patients with pT1 bladder cancer is currently conducted to validate our findings.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPARATIVE GENOMIC HYBRIDIZATION; CHROMOSOMAL INSTABILITY; SUSCEPTIBILITY GENE; EXPRESSION; ASSOCIATION; IMBALANCES; CARCINOMAS; ANEUPLOIDY; P53; bladder cancer; histologically benign urothelium; fluorescence in situ hybridization; STK15; SNP analysis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Onkogenomik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 08:15 |
| Last Modified: | 01 Dec 2020 08:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32131 |
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