Friedrich, Juergen and Eder, Wolfgang and Castaneda, Juana and Doss, Markus and Huber, Elisabeth and Ebner, Reinhard and Kunz-Schughart, Leoni A. (2007) A reliable tool to determine cell viability in complex 3-D culture: The acid phosphatase assay. JOURNAL OF BIOMOLECULAR SCREENING, 12 (7). pp. 925-937. ISSN 1087-0571,
Full text not available from this repository. (Request a copy)Abstract
Cell-based assays are more complex than cell-free test systems but still reflect a highly artificial cellular environment. Incorporation of organotypic 3-dimensional (3-D) culture systems into mainstream drug development processes is increasingly discussed but severely limited by complex methodological requirements. The objective of this study was to explore a panel of standard assays to provide an easy-handling, standardized protocol for rapid routine analysis of cell survival in multicellular tumor spheroid-based antitumor drug testing. Spheroids of 2 colon carcinoma cell lines were characterized for evaluation. One of the assay systems tested could reliably be used to determine cell viability in spheroids. The authors verified that the acid phosphatase assay (APH) is applicable for single spheroids in 96-well plates, does not require spheroid dissociation, and is linear and highly sensitive for HT29 and HCT-116 spheroids up to diameters of 650 mu m and 900 gm, consisting of 40,000 and 80,000 cells, respectively. Treatment of HT29 and HCT-116 cells with 5-fluorouracil, Irinotecan, and C-1311 revealed critically reduced drug efficacies in 3-D versus monolayer culture, which is discussed in light of literature data. The experimental protocol presented herein is a small but substantial contribution to the establishment of sophisticated 3-D in vitro systems in the antitumor drug screening scenario.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENHANCED ANTITUMOR-ACTIVITY; COLON-CARCINOMA CELLS; MULTICELLULAR SPHEROIDS; GROWTH-INHIBITION; IN-VITRO; PRECLINICAL EVALUATION; ANTINEOPLASTIC AGENTS; COLORECTAL-CANCER; MONOLAYER-CULTURE; TUMOR SPHEROIDS; cell-based assay; 3-D culture; spheroid; antitumor drug testing; cell viability |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 08:22 |
| Last Modified: | 01 Dec 2020 08:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32135 |
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