Lehle, Karla and Kunz-Schughart, Leoni A. and Kuhn, Peter and Schreml, Stephan and Birnbaum, Dietrich E. and Preuner, Juergen G. (2007) Validity of a patient-derived system of tissue-specific human endothelial cells: interleukin-6 as a surrogate marker in the coronary system. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 293 (3). H1721-H1728. ISSN 0363-6135,
Full text not available from this repository. (Request a copy)Abstract
The aim of our study was to evaluate the relevance of tissue- and species-specific endothelial cells (EC) to study EC-dependent mechanisms in inflammatory-mediated tissue injury. We established an isolation protocol for highly purified EC (pEC) preparations of different origin and compared EC-specific inflammatory responses. Fluorescence-activated cell separation was used to obtain pEC cultures from different human arterial ( coronary artery, internal thoracic artery) and venous ( umbilical vein, saphenous vein) vessels. All pEC were analyzed for growth kinetics, morphology, release of cytokines/chemokines, and expression of E-selectin. For all different EC cultures, purities of >= 99% were reproducibly achieved. The EC isolation did not affect EC growth, morphology, and function. However, characterization of pEC from different vessel materials revealed an intrinsic, tissue- specific functional heterogeneity of EC cultures. Despite an arterial and venous difference in the secretion of IL-8 and monocyte chemoattractant protein-1, especially EC from coronary arteries produced significantly more IL-6 compared with other EC types, independent of age, gender, and disease of the cell donors. In contrast, the expression of E-selectin was not affected. We conclude that the proposed isolation protocol allows the generation of a pEC bank, enabling us to study tissue- specific aspects at the level of the endothelium.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROGENITOR CELLS; HEART-FAILURE; IN-VITRO; CULTURE; EXPRESSION; PATHOPHYSIOLOGY; TRANSPLANTATION; PHYSIOLOGY; CYTOKINES; RELEASE; purification; cell growth; fluorescence-activated cell separation; function |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 11:44 |
| Last Modified: | 01 Dec 2020 11:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32224 |
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