Minuth, Will W. and Denk, Lucia and Hui, Kanghong and Castrop, Hayo and Gomez-Sanchez, Celso (2007) The tubulogenic effect of aldosterone is attributed to intact binding and intracellular response of the mineralocorticoid receptor. CENTRAL EUROPEAN JOURNAL OF BIOLOGY, 2 (3). pp. 307-325. ISSN 1895-104X,
Full text not available from this repository. (Request a copy)Abstract
Little is known about the extra- and intracellular stimuli inducing, renal stem/progenitor cells to develop into three-dimensionally structured tubules. To study this specific development in a controlled environment, we used an advanced culture technique. Embryonic tissue derived from neonatal rabbit kidney was placed in a perfusion culture container at the interface of an artificial interstitium made of a polyester fleece. Culture was carried out in chemically defined Iscove's Modified Dulbecco's Medium (IMDM) for 13 days. Development of tubules was histochemically detected on cryosections labeled with Soybean Agglutinin (SBA). The experiments showed that aldosterone exerts a specific tubulouenic effect. Application of aldosterone (1 x 10(-7) M) raised numerous SBA-labeled tubules, while in the absence of the steroid hormone the development of tubules was lacking. Specificity of hormone action was analyzed by the use of aldosterone antagonists. Administration of spironolactone (1 x 10(-4) M) and canrenoate (1 x 10(-5) M) completely inhibited the development of tubules. Finally, disrupting the intracellular molecular complex of the mineralocorticoid receptor (MR) and heat shock proteins by geldanamycin (2 mu g/ml) prevented the development of tubules. Our results sug est that the tubulogenic effect induced by aldosterone is attributed to both hormone binding and an undisturbed intracellular response of the MR. (c) Versita Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEAT-SHOCK-PROTEIN; ARTIFICIAL INTERSTITIUM; STEM-CELLS; RENAL TUBULES; KIDNEY; DIFFERENTIATION; CULTURE; GROWTH; TISSUE; stem/progenitor cells; aldosterone; mineralocorticoid receptor; perfusion culture; artificial interstitium; kidney |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 08:49 |
| Last Modified: | 01 Dec 2020 08:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32233 |
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