van Oers, Johanna M. M. and Wild, Peter J. and Buryer, Maximilian and Denzinger, Stefan and Stoehr, Robert and Rosskopf, Elke and Hofstaedter, Ferdinand and Steyerberg, Ewout W. and Klinkhammer-Schalke, Monika and Zwarthoff, Ellen C. and van der Kwast, Theodorus H. and Hartmann, Arndt (2007) FGFR3 mutations and a normal CK20 staining pattern define low-grade noninvasive urothelial bladder tumours. EUROPEAN UROLOGY, 52 (3). pp. 760-768. ISSN 0302-2838,
Full text not available from this repository. (Request a copy)Abstract
Objectives: Molecular markers superior to conventional clinicopathologic parameters are needed to predict disease courses in bladder cancer patients. In this study, we investigated four markers (Ki-67, TP53, CK20, FGFR3) in primary urothelial bladder tumours and compared them with traditional pathologic features. Methods: Tissue microarrays were used to analyse CK20, TP53, and Ki-67 expression immunohistochemically in 255 unselected patients. FGFR3 mutations were detected by SNaPshot analysis. Results: Abnormal CK20 expression was strongly associated with higher tumour grades and stages (p < 0.001); however, 65% of pTa tumours revealed an abnormal CK20 pattern. In the group of pTaG1 tumours, 59% presented with an abnormal CK20 pattern, whereas 82% carried the FGFR3 mutation. In the group of bladder tumours with normal CK20 pattern, the FGFR3 gene was mutated in 89%, whereas a mutated FGFR3 gene was found in only 37% of cases with abnormal CK20 expression (p < 0.001). All markers proved to be strong predictors of disease-specific survival in univariate studies. However, in multivariate analyses they were not independent from classical pathologic parameters. None of the molecular markers was significantly associated with tumour recurrence. Conclusions: Dysregulation of CK20 expression is an early event in the carcinogenesis of papillary noninvasive bladder cancer, but occurs later than FGFR3 mutations. The group of low-grade noninvasive papillary tumours is defined by the presence of an FGFR3 mutation and a normal CK20 expression pattern. (c) 2007 European Association of Urology. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR RECEPTOR-3; TRANSITIONAL-CELL CARCINOMAS; IN-SITU; CYTOKERATIN-20 EXPRESSION; PROLIFERATION INDEX; PREDICT RECURRENCE; CANCER; P53; CLASSIFICATION; PROGNOSIS; bladder cancer; CK20; FGFR3; Ki-67; molecular markers; TP53 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie Medicine > Lehrstuhl für Urologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 12:01 |
| Last Modified: | 08 Apr 2021 05:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32276 |
Actions (login required)
 |
View Item |
