Schilling, Elinar and Rehli, Michael (2007) Global, comparative analysis of tissue-specific promoter CpG methylation. GENOMICS, 90 (3). pp. 314-323. ISSN 0888-7543,
Full text not available from this repository. (Request a copy)Abstract
Understanding cell-type-specific epigenetic codes on a global level is a major challenge after the sequencing of the human genome has been completed. Here we applied methyl-CpG immunoprecipitation (MCIp) to obtain comparative methylation profiles of coding and noncoding genes in three human tissues, testis, brain, and monocytes. Forty-four mainly testis-specific promoters were independently validated using bisulfite sequencing or single-gene MCIp, confirming the results obtained by the MCIp microarray approach. We demonstrate the previously unknown somatic hypermethylation at many CpG-rich, testis-specific gene promoters, in particular in ampliconic areas of the Y chromosome. We also identify a number of miRNA genes showing tissue-specific methylation patterns. The comparison of the obtained tissue methylation profiles with corresponding gene expression data indicates a significant association between tissue-specific promoter methylation and gene expression, not only in CpG-rich promoters. In summary, our study highlights the exceptional epigenetic status of germ-line cells in testis and provides a global insight into tissue-specific DNA methylation patterns. (c) 2007 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA METHYLATION; GENE-EXPRESSION; CANCER-CELLS; GERM-LINE; MAMMALIAN DEVELOPMENT; X-CHROMOSOME; Y-CHROMOSOME; BORIS; CTCF; DEREPRESSION; DNA methylation; epigenetics; gene regulation; methylation profiling |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2020 10:05 |
| Last Modified: | 02 Dec 2020 10:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32281 |
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