Goritz, Christian and Thiebaut, Renaud and Tessier, Luc-Henri and Nieweg, Katja and Moehle, Christoph and Buard, Isabelle and Dupont, Jean-Luc and Schurgers, Leon J. and Schmitz, Gerd and Pfrieger, Frank W. (2007) Glia-induced neuronal differentiation by transcriptional regulation. GLIA, 55 (11). pp. 1108-1122. ISSN 0894-1491, 1098-1136
Full text not available from this repository. (Request a copy)Abstract
There is increasing evidence that different phases of brain development depend on neuron-glia interactions including postnatal key events like synaptogenesis. To address how glial cells influence synapse development, we analyzed whether and how glia-derived factors affect gene expression in primary cultures of immunoisolated rat retinal ganglion cells (RGCs) by oligonucleotide microarrays. Our results show that the transcript pattern matched the developmental stage and characteristic properties of RGCs in vitro. Glia-conditioned medium (GCM) and cholesterol up- and downregulated a limited number of genes that influence the development of dendrites and synapses and regulate cholesterol and fatty acid metabolism. The oligonucleotide microarrays detected the transcriptional regulation of neuronal cholesterol homeostasis in response to GCM and cholesterol treatment. Surprisingly, our study revealed neuronal expression and glial regulation of matrix gla protein (Mgp). Together, our results suggest that glial cells promote different aspects of neuronal differentiation by regulating transcription of distinct classes of genes. (C) 2007 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RETINAL GANGLION-CELLS; MATRIX GLA PROTEIN; CENTRAL-NERVOUS-SYSTEM; SYNAPSE FORMATION; CNS SYNAPTOGENESIS; GENE-EXPRESSION; HIPPOCAMPAL-NEURONS; NEUROTROPHIC FACTOR; MEMBRANE-PROTEIN; BINDING PROTEIN; microarray analysis; retinal ganglion cells; cholesterol; gene expression profiling; Keutel syndrome |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 14:08 |
| Last Modified: | 01 Dec 2020 14:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32387 |
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