Feliciangeli, Sylvain and Bendahhou, Said and Sandoz, Guillaume and Gounon, Pierre and Reichold, Markus and Warth, Richard and Lazdunski, Michel and Barhanin, Jacques and Lesage, Florian (2007) Does sumoylation control K2P1/TWIK1 background K+ channels? CELL, 130 (3). pp. 563-569. ISSN 0092-8674, 1097-4172
Full text not available from this repository. (Request a copy)Abstract
A novel model for the regulation of cell excitability has recently been proposed. It originates from the observation that the background K+ channel K2P1 (TWIK1) may be silenced by sumoylation in Xenopus oocytes and that inactivation of the putative sumoylation site ( mutation K274E) gives rise to robust current expression in transfected COS-7 cells. Here, we show that only the mutation K274E, and not K274R, is associated with an increase of K2P1 current density, suggesting a charge effect of K274E. Furthermore, we failed to observe any band shift by western blot analysis that would confirm an eventual sumoylation of K2P1 in COS-7 cells and oocytes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POTASSIUM CHANNELS; TWIK-1; PHARMACOLOGY; EXPRESSION; SUMO-1; KIDNEY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Richard Warth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2020 14:21 |
| Last Modified: | 01 Dec 2020 14:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32394 |
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