Dettmer, K. and Hanna, D. and Whetstone, P. and Hansen, R. and Hammock, B. D. (2007) Autism and urinary exogenous neuropeptides: development of an on-line SPE-HPLC-tandem mass spectrometry method to test the opioid excess theory. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 388 (8). pp. 1643-1651. ISSN 1618-2642,
Full text not available from this repository. (Request a copy)Abstract
Autism is a complex neurodevelopmental disorder with unknown etiology. One hypothesis regarding etiology in autism is the "opioid peptide excess" theory that postulates that excessive amounts of exogenous opioid-like peptides derived from dietary proteins are detectable in urine and that these compounds may be pathophysiologically important in autism. A selective LC-MS/MS method was developed to analyze gliadinomorphin, beta-casomorphin, deltorphin 1, and deltorphin 2 in urine. The method is based on on-line SPE extraction of the neuropeptides from urine, column switching, and subsequent HPLC analysis. A limit of detection of 0.25 ng/mL was achieved for all analytes. Analyte recovery rates from urine ranged between 78% and 94%, with relative standard deviations of 0.2-6.8%. The method was used to screen 69 urine samples from children with and without autism spectrum disorders for the occurrence of neuropeptides. The target neuropeptides were not detected above the detection limit in either sample set.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SPECTRUM DISORDER; CHILDREN; PEPTIDES; autism; neuropeptides; beta-casomorphin; gliadinomorphin; opioid peptide excess theory; on-line SPE-HPLC-MS/MS |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2020 06:31 |
| Last Modified: | 02 Dec 2020 06:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32409 |
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