Pisa, Kim Y. and Huber, Harald and Thomm, Michael and Mueller, Volker (2007) A sodium ion-dependent A(1)A(o) ATP synthase from the hyperthermophilic archaeon Pyrococcus furiosus. FEBS JOURNAL, 274 (15). pp. 3928-3938. ISSN 1742-464X,
Full text not available from this repository. (Request a copy)Abstract
The rotor subunit c of the A(1)A(0) ATP synthase of the hyperthermophilic archaeon Pyrococcus furiosus contains a conserved Na+-binding motif, indicating that Na+ is a coupling ion. To experimentally address the nature of the coupling ion, we isolated the enzyme by detergent solubilization from native membranes followed by chromatographic separation techniques. The entire membrane-embedded motor domain was present in the preparation. The rotor subunit c was found to form an SDS-resistant oligomer. Under the conditions tested, the enzyme had maximal activity at 100 degrees C, had a rather broad pH optimum between pH 5.5 and 8.0, and was inhibited by diethystilbestrol and derivatives thereof. ATP hydrolysis was strictly dependent on Na+, with a Km of 0.6 mm. Li+, but not K+, could substitute for Na+. The Na+ dependence was less pronounced at higher proton concentrations, indicating competition between Na+ and H+ for a common binding site. Moreover, inhibition of the ATPase by N',N'-di-cyclohexylcarbodiimide could be relieved by Na+. Taken together, these data demonstrate the use of Na+ as coupling ion for the A(1)A(0) ATP synthase of Pyrococcus furiosus, the first Na+ A(1)A(0) ATP synthase described.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARBODIIMIDE-REACTIVE PROTEIN; NA+-TRANSLOCATING F1F0-ATPASE; METHANOSARCINA-MAZEI GO1; C SUBUNIT OLIGOMER; ESCHERICHIA-COLI; ACETOBACTERIUM-WOODII; F-TYPE; PROPIONIGENIUM-MODESTUM; ILYOBACTER-TARTARICUS; CLOSTRIDIUM-PARADOXUM; A(1)A(o) ATP synthase; Archaea; hyperthermophile; Na+; Pyrococcus |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2020 07:11 |
| Last Modified: | 02 Dec 2020 07:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32442 |
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