Schifferer, Rainer and Liebisch, Gerhard and Bandulik, Sascha and Langmann, Thomas and Dada, Ashraf and Schmitz, Gerd (2007) ApoA-I induces a preferential efflux of monounsaturated phosphatidylcholine and medium chain sphingomyelin species from a cellular pool distinct from HDL3 mediated phospholipid efflux. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1771 (7). pp. 853-863. ISSN 1388-1981,
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Electrospray ionization tandem mass spectrometry (ESI-MS/MS) was used for a detailed analysis of cellular phospholipid and cholesterol efflux in free cholesterol (FC) loaded human primary fibroblasts and human monocyte-derived macrophages (HMDM) loaded with enzymatically modified LDL (E-LDL). Although both cell models differed significantly in their cellular lipid composition, a higher apoA-I specific efflux was found for monounsaturated phosphatidylcholine (PC) species together with a decreased contribution of polyunsaturated PC species in both cell types. Moreover, medium chain sphingomyelin (SPM) species SPM 14:0 and SPM 16:1 were translocated preferentially to apoA-I in both cell types. in contrast to fibroblasts, HMDM displayed a considerable proportion of cholesteryl esters (CE) in basal and apoA-I specific efflux media, most likely due to secretion of CE associated to apoE. Analysis of HDL3 mediated lipid efflux from HMDM using D-9-choline and C-13(3)-FC stable isotope labeling revealed significantly different D-9-PC and D-9-SPM species pattern for apoA-I and HDL3 specific efflux media, which indicates a contribution of distinct cellular phospholipid pools to apoA-I and HDL3 mediated efflux. Together with a partial loading of fibroblasts and HMDM with HDL3-derived CE species, these data add further evidence for retroendocytosis of HDL. In summary, analysis of apoA-I/ABCA1 and HDL3 mediated lipid efflux by ESI-MS/MS demonstrated a preferential efflux of monounsaturated PC and medium chain SPM to apoA-I. Moreover, this is the first study, which provides evidence for distinct cellular phospholipid pools used for lipid transfer to apoA-I and HDL3 from the analysis of phospholipid species pattern in HMDM. (c) 2007 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH-DENSITY-LIPOPROTEIN; TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; MONOCYTE-DERIVED MACROPHAGES; SCAVENGER RECEPTOR BI; APOLIPOPROTEIN-A-I; CASSETTE TRANSPORTER 1; CHOLESTEROL EFFLUX; TANGIER-DISEASE; UNESTERIFIED CHOLESTEROL; tandem mass spectrometry; stable isotope labeling; electrospray ionization; quantitative lipid analysis; lipidomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2020 11:34 |
| Last Modified: | 02 Dec 2020 11:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32520 |
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