Murphy, Matthew B. and Hartgerink, Jeffrey D. and Goepferich, Achim and Mikos, Antonios G. (2007) Synthesis and in vitro hydroxyapatite binding of peptides conjugated to calcium-binding moieties. BIOMACROMOLECULES, 8 (7). pp. 2237-2243. ISSN 1525-7797,
Full text not available from this repository. (Request a copy)Abstract
To confer bone-binding properties to proteins and other biological agents that lack specific targeting capacity, model peptide-based molecules were synthesized containing poly(aspartic acid), poly(glutamic acid), or a bisphosphonate (pamidronate). These motifs have well-documented affinities to hydroxyapatite, a property desirable for the targeting of molecules to bone for drug delivery and tissue engineering applications. Model peptides of increasing molecular mass (5-33 amino acids) were directly conjugated to eight aspartic acids (Asp(8)), eight glutamic acids (Glu(8)), or pamidronate, purified by high-performance liquid chromatography, and characterized by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectroscopy. The modified peptides were incubated with hydroxyapatite in phosphate-buffered saline at physiological conditions over 24 h. This study revealed a significant amount (> 90%) of conjugated peptides adsorbed to the hydroxyapatite as compared to unmodified peptides (< 5%). It was found that while there were significant differences between the different hydroxyapatite-binding and control groups for all time points, the size of the peptide had no statistical effect on peptide-hydroxyapatite binding. These results demonstrate that bisphosphonate and oligopeptide conjugates hold great promise for the development of new bioactive molecules for bone-specific applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DRUG-DELIVERY SYSTEMS; BONE AFFINITY; ALENDRONATE; BISPHOSPHONATES; MICE; VIVO; |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2020 11:36 |
| Last Modified: | 02 Dec 2020 11:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32522 |
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