Obermann, Ellen C. and Went, Philip and Tzankov, Alexandar and Pileri, Stefano A. and Hofstaedter, Ferdinand and Marienhagen, Joerg and Stoehr, Robert and Dirnhofer, Stephan (2007) Cell cycle phase distribution analysis in chronic lymphocytic leukaemia: a significant number of cells reside in early G1-phase. JOURNAL OF CLINICAL PATHOLOGY, 60 (7). pp. 794-797. ISSN 0021-9746,
Full text not available from this repository. (Request a copy)Abstract
Background and Aims: Chronic lymphocytic leukaemia (CLL) is a frequent non-Hodgkin lymphoma characterised by a heterogeneous clinical course. Assessment of cell cycle phase kinetics might be important for prediction of clinical behaviour and prognosis. Methods: Distribution of neoplastic cells in CLL within the cell cycle was evaluated by determining the labelling indices (LI, i.e. percentage of positive cells) of markers specific for late G1-phase (cyclin E), S-phase (cyclin A), and G2/M-phase (cyclin B1), and Mcm2, a novel marker of proliferative potential, in a large cohort of patients (n = 79) using tissue microarray (TMA) technology. Utilising a combination of these markers, an algorithm was developed-subtracting the combined LIs of cyclin E, cyclin A and cyclin B1 from the LI of Mcm2-to determine the percentage of tumour cells residing in early G1-phase, which is probably a critical state for the malignant potential of CLL. Results: 27.11% of cells had acquired proliferative potential as indicated by expression of Mcm2. Only a small number of cells were found to be in late G1-phase (7.16%), S-phase (3.31%) or G2/M-phase (0.98%), while 15.66% of cells were considered to be in early G1-phase. Conclusion: Cell cycle phase distribution can easily be assessed by immunohistochemistry in routinely processed paraffin-embedded specimens. A large number of neoplastic cells in CLL have proliferative potential, with a significant sub-population residing in early G1-phase. Estimates of these cells may identify cases likely to exhibit a more aggressive biological behaviour and adverse clinical course.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TISSUE MICROARRAY ANALYSIS; DNA-REPLICATION; IMMUNOHISTOCHEMICAL METHOD; PROGNOSTIC-SIGNIFICANCE; HUMAN TUMORS; EXPRESSION; LYMPHOMA; PROTEINS; KI-67; KI67; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie Medicine > Abteilung für Nuklearmedizin Medicine > Lehrstuhl für Urologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2020 12:14 |
| Last Modified: | 02 Dec 2020 12:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32553 |
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