Baier, Juergen and Fuss, Thomas and Poellmann, Claudia and Wiesmann, Christopher and Pindl, Katrin and Engl, Roland and Baumer, Daniela and Maier, Max and Landthaler, Michael and Baeumler, Wolfgang (2007) Theoretical and experimental analysis of the luminescence signal of singlet oxygen for different photosensitizers. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 87 (3). pp. 163-173. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
After the generation by different photosensitizers, the direct detection of singlet oxygen is performed by measuring its luminescence at 1270 nm. Using, an infrared sensitive photomultiplier, the complete rise and decay time of singlet oxygen luminescence is measured at different concentrations of a photosensitizer, quencher, or oxygen. This allows the extraction of important information about the photosensitized generation of singlet oxygen and its decay, in particular at different oxygen concentrations. Based on theoretical considerations all important relaxation rates and rate constants were determined for the triplet T, states of the photosensitizers and for singlet oxygen. In particular, depending on the oxygen or quencher concentration, the rise or the decay time of the luminescence signal exhibit different meanings regarding the lifetime of singlet oxygen or triplet T-1-state. To compare with theory, singlet oxygen was generated by nine different photosensitizers dissolved in either H2O, D2O or EtOD. When using H2O as solvent, the decaying part of the luminescence signal is frequently not the lifetime of singlet oxygen, in particular at low oxygen concentration. Since cells show low oxygen concentrations, this must have an impact when looking at singlet oxygen detection in vitro or in vivo. (c) 2007 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEAR-INFRARED LUMINESCENCE; TIME-RESOLVED DETECTION; PHOTODYNAMIC THERAPY; MOLECULAR-OXYGEN; RATE CONSTANTS; IN-VITRO; GENERATION; PHOTOFRIN; CELLS; SUSPENSIONS; singlet oxygen; luminescence at 1270 nm; lifetime; variation of oxygen concentration; quencher; rise rate; decay rate |
| Subjects: | 500 Science > 530 Physics 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Physics > Institute of Experimental and Applied Physics |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Dec 2020 13:16 |
| Last Modified: | 07 Dec 2020 13:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32596 |
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