Weissinger, Eva M. and Schiffer, Eric and Hertenstein, Bernd and Ferrara, James L. and Holler, Ernst and Stadler, Michael and Kolb, Hans-Jochem and Zander, Axel and Zurbig, Petra and Kellmann, Markus and Ganser, Arnold (2007) Proteomic patterns predict acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation. BLOOD, 109 (12). pp. 5511-5519. ISSN 0006-4971, 1528-0020
Full text not available from this repository. (Request a copy)Abstract
Acute graft-versus-host disease (aGvHD) contributes significantly to morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Diagnosis of GvHD is mainly based on clinical features and tissue biopsies. A noninvasive, unbiased laboratory test for GvHD diagnosis does not exist. Here we describe the application of capillary electrophoresis coupled online with mass spectrometry (CE-MS) to 13 samples from 10 patients with aGvHD of grade 11 or more and 50 control samples from 23 patients without GvHD. About 170 GvHD-specific polypeptides were detected and a tentatively aGvHD-specific model consisting of 31 polypeptides was chosen, allowing correct classification of 13 of 13 (sensitivity 100.0% [95% confidence interval {CI} 75.1 to 100.0]) aGvHD samples and 49 of 50 (specificity 98.0% [95% CI 89.3 to 99.7]) control samples of the training set. The subsequent blinded evaluation of 599 samples enabled diagnosis of aGvHD greater than grade II, even prior to clinical diagnosis, with a sensitivity of 83.1% (95% CI 73.1 to 87.9) and a specificity of 75.6% (95% CI 71.6 to 79.4). Thus, high-resolution proteome analysis represents an unbiased laboratory-based screening method, enabling diagnosis, and possibly enabling preemptive therapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACUTE MYELOID-LEUKEMIA; CAPILLARY-ELECTROPHORESIS; MASS-SPECTROMETRY; DIAGNOSTIC PURPOSES; FOLLOW-UP; PROTEIN; SERUM; BIOMARKERS; SEQUENCE; PATHOPHYSIOLOGY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Dec 2020 13:24 |
| Last Modified: | 07 Dec 2020 13:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32609 |
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