Fernandes, Andre T. and Soares, Claudio M. and Pereira, Manuela M. and Huber, Robert and Grass, Gregor and Martins, Ligia O. (2007) A robust metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus. FEBS JOURNAL, 274 (11). pp. 2683-2694. ISSN 1742-464X, 1742-4658
Full text not available from this repository. (Request a copy)Abstract
The gene, Aquifex aeolicus AAC07157.1, encoding a multicopper oxidase (McoA) and localized in the genome as part of a putative copper-resistance determinant, has been cloned, over-expressed in Escherichia coli and the recombinant enzyme purified to homogeneity. The purified enzyme shows spectroscopic and biochemical characteristics typical of the well-characterized multicopper oxidase family of enzymes. McoA presents higher specificity (k(cat)/K-m) for cuprous and ferrous ions than for aromatic substrates and is therefore designated as a metallo-oxidase. Addition of copper is required for maximal catalytic efficiency. A comparative model structure of McoA has been constructed and a striking structural feature is the presence of a methionine-rich region (residues 321-363), reminiscent of those found in copper homeostasis proteins. The kinetic properties of a mutant enzyme, McoA Delta P321-V363, deleted in the methionine-rich region, provide evidence for the key role of this region in the modulation of the catalytic mechanism. McoA has an optimal temperature of 75 degrees C and presents remarkable heat stability at 80 and 90 degrees C, with activity lasting for up to 9 and 5 h, respectively. McoA probably contributes to copper and iron homeostasis in A. aeolicus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONFERS COPPER TOLERANCE; MULTICOPPER OXIDASE; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; ENDOSPORE COAT; CRYSTAL-STRUCTURE; CUEO; LACCASE; SPECTROSCOPY; COMPONENT; Aquifex aeolicus; copper and iron homeostasis; hyperthermophilic bacteria; metallo-oxidase; multicopper oxidases |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Dec 2020 08:29 |
| Last Modified: | 04 Dec 2020 08:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32655 |
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