Brenmoehl, Julia and Lang, Markus and Hausmann, Martin and Leeb, Sandra N. and Falk, Werner and Schoelmerich, Juergen and Goeke, Michael and Rogler, Gerhard (2007) Evidence for a differential expression of fibronectin splice forms ED-A and ED-B in Crohn's disease (CD) mucosa. INTERNATIONAL JOURNAL OF COLORECTAL DISEASE, 22 (6). pp. 611-623. ISSN 0179-1958,
Full text not available from this repository. (Request a copy)Abstract
Background and aims Fibronectin (FN) is an essential factor for the induction of migration of primary colonic lamina propria fibroblasts (CLPF). The FN isoform ED-A is an important inducer of migration. Recently, we have shown that CLPF isolated from inflamed Crohn's disease (CD) mucosa migrated significantly less than control CLPF. We, therefore, investigated changes in IN or integrin expression that could be relevant for CLPF migration. Materials and methods mRNA of control-CLPF and CLPF isolated from fibrotic mucosa of CD patients was subtractively hybridized. Expression of FN, ED-A, and ED-B in frozen sections from intestinal mucosa was determined by immunohistochemistry. The mRNA expression of the FN isoforms in control, CD, and fibrosis biopsies was quantified by real-time polymerase chain reaction (PCR). Integrin alpha 5 beta 1 protein and mRNA expression was analyzed by fluorescence activated cell sorting (FACS) and PCR, respectively. Results Subtractive hybridization indicated differential regulation of FN isoform expression in CD. The immunohistochemical analysis of FN protein revealed a reduction of FN isoforrns in inflamed CD mucosa compared to control mucosa. In CD fistulae, the ED-A and ED-B isoforms were virtually absent. In fibrotic mucosa, both proteins were increased. Real-time PCR showed a decrease of FN and ED-A expression during mucosal inflammation in CD in contrast to UC and a significant increase of FN and isoforms in CD fibrosis. No difference was found for protein and mRNA of integrin alpha 5 beta 1 in control, CD, and fibrosis CLPF by FACS and PCR. Conclusion Downregulated expression of migration-inducing FN-isoforrns in contrast to unchanged FN receptor expression may contribute to the observed alterations of CD CLPF migration.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELLULAR FIBRONECTIN; MESSENGER-RNAS; FIBROBLASTS; ADHESION; MIGRATION; SEGMENT; DOMAIN; ALPHA; RAT; MODULATION; fibronectin isoforms; integrin alpha 5 beta 1; colonic lamina propria fibroblasts; migration; fibrosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Dec 2020 10:04 |
| Last Modified: | 03 Dec 2020 10:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32667 |
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