Treeck, Oliver and Pfeiler, Georg and Mitter, Diana and Lattrich, Claus and Piendl, Gerhard and Ortmann, Olaf (2007) Estrogen receptor beta 1 exerts antitumoral effects on SK-OV-3 ovarian cancer cells. JOURNAL OF ENDOCRINOLOGY, 193 (3). pp. 421-433. ISSN 0022-0795,
Full text not available from this repository. (Request a copy)Abstract
Estrogen receptor (ER) beta 1 and its splice variants are expressed both in ovary and ovarian cancer. We studied the role of ER beta 1 and two of its splice variants in regulation of gene expression, cellular proliferation, apoptosis, and migration of an ovarian cancer cell line. In this study, we transfected SK-OV-3 ovarian cancer cells with vectors coding for ER beta 1 or its splice variants ER beta-delta 125 and ER beta-delta 1256, and tested their response to estrogen and tamoxifen in comparison with the untransfected cells. Heterologous expression of ER beta 1, but not of the exon-deleted ER beta variants resulted in notably slower cell growth of SK-OV-3 ovarian cancer cells, an effect accompanied by more than tenfold increase of cyclin-dependent kinase inhibitor p21(WAF1) transcript levels and a significant reduction of cyclin A2 mRNA levels. SK-OV-3 cells stably overexpressing ER beta 1 ligand independently also exhibited an increased apoptosis rate and a significantly decreased motility, an effect accompanied by upregulation of fibulin 1c. Our data demonstrate that ER beta 1, but not the exon-deleted isoforms tested exerts multiple antitumoral effects on SK-OV-3 ovarian cancer cells even in the absence of estradiol or functional ER alpha.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESTROGEN-RECEPTOR-BETA; ALPHA ER-ALPHA; MESSENGER-RNA; BREAST-CANCER; DIFFERENTIAL EXPRESSION; PROGESTERONE-RECEPTOR; REPLACEMENT THERAPY; SPLICE VARIANTS; IDENTIFICATION; PROLIFERATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Geburtshilfe) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Dec 2020 08:59 |
| Last Modified: | 04 Dec 2020 08:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32679 |
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