Baier, Juergen and Maisch, Tim and Maier, Max and Landthaler, Michael and Baeumler, Wolfgang (2007) Direct detection of singlet oxygen generated by UVA irradiation in human cells and skin. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 127 (6). pp. 1498-1506. ISSN 0022-202X, 1523-1747
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UVA light produces deleterious biological effects in which singlet oxygen plays a major role. These effects comprise a significant risk of carcinogenesis in the skin and cataract formation of the eye lens. Singlet oxygen is generated by UVA light absorption in endogenous molecules present in the cells. To elucidate the primary processes and sources of singlet oxygen in tissue, it is a major goal to uncover the hidden process of singlet oxygen generation, in particular in living tissue. When exposing keratinocytes or human skin in vivo to UVA laser light (355 nm) at 6 J/cm(2), we measured the luminescence of singlet oxygen at 1,270 nm. This is a positive and direct proof of singlet oxygen generation in cells and skin by UVA light. Moreover, a clear signal of singlet oxygen luminescence was detected in phosphaticlylcholine suspensions (water or ethanol) irradiated by UVA. Oxidized products of phosphatidylcholine are the likely chromophores because phosphatidylcholine itself does not absorb at 355 nm. The signal intensity was reduced by mannitol or super oxide dismutase. Additionally, the monochromatic UVA irradiation at 355 nm leads to upregulation of the key cytokine IL-12. This affects the balance of UV radiation on the immune system, which is comparable to effects of broadband UVA irradiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ULTRAVIOLET-A RADIATION; MOLECULAR-OXYGEN; IN-VITRO; PHOTODYNAMIC THERAPY; DERMAL FIBROBLASTS; GENE-EXPRESSION; LIGHT-A; ACTIVATION; EXPOSURE; KERATINOCYTES; |
| Subjects: | 500 Science > 530 Physics 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Physics > Institute of Experimental and Applied Physics |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Dec 2020 09:21 |
| Last Modified: | 04 Dec 2020 09:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32684 |
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