Liu, Wei and Vierke, Gudrun and Wenke, Ann-Kathrin and Thomm, Michael and Ladenstein, Rudolf (2007) Crystal structure of the archaeal heat shock regulator from Pyrococcus furiosus: A molecular chimera representing eukaryal and bacterial features. JOURNAL OF MOLECULAR BIOLOGY, 369 (2). pp. 474-488. ISSN 0022-2836, 1089-8638
Full text not available from this repository. (Request a copy)Abstract
We report here the crystal structure of a protein from Pyrococcus furiosus (Phr) that represents the first characterized heat shock transcription factor in archaea. Phr specifically represses the expression of heat shock genes at physiological temperature in vitro and in vivo but is released from the promoters upon heat shock response. Structure analysis revealed a stable homodimer, each subunit consisting of an N-terminal winged helix DNAbinding domain (wH-DBD) and a C-terminal antiparallel coiled coil helical domain. The overall structure shows as a molecular chimera with significant folding similarity of its DBD to the bacterial SmtB/ArsR family, while its C-terminal part was found to be a remote homologue of the eukaryotic BAG domain. The dimeric protein recognizes a palindromic DNA sequence. Molecular docking and mutational analyses suggested a novel binding mode in which the major specific contacts occur at the minor groove interacting with the strongly basic wing containing a cluster of three arginine residues. (C) 2007 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-STRUCTURE ALIGNMENT; DNA-BINDING PROTEIN; TRANSCRIPTIONAL REGULATION; DENSITY MODIFICATION; COILED COILS; IN-VIVO; FAMILY; COMPLEX; DOMAIN; REFINEMENT; archaea; Phr; heat shock; transcriptional regulation; winged helix |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Dec 2020 09:31 |
| Last Modified: | 04 Dec 2020 09:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32688 |
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