Lehle, Karla and Haubner, Frank and Muenzel, Daniela and Birnbaum, Dietrich E. and Preuner, Juergen G. (2007) Development of a disease-specific model to evaluate endothelial dysfunction in patients with diabetes mellitus. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 357 (1). pp. 308-313. ISSN 0006-291X,
Full text not available from this repository. (Request a copy)Abstract
Diabetic patients have an increased cardiovascular risk. We propose to characterize the endothelial dysfunction in a disease-specific in vitro model. Human saphenous vein endothelial cells (HSVEC) were isolated from coronary artery bypass patients without and with non-insulin-dependent diabetes mellitus. Growth kinetics and proinflammatory responses (expression of adhesion molecules, cytokines) were documented under non-stimulating conditions. Diabetic HSVEC showed delayed growth kinetics with reduced cell densities of about 40%. During exponential growth of diabetic EC, the surface expression of adhesion molecules was increased 10-fold (p <= 0.05). However, in a monolayer the expression adapted to low levels of non-diabetic EC. In addition, diabetic EC produced significantly more soluble E-selectin, VCAM-1, IL-6 and MCP-1. Our results suggest a link between the pathologically proinflammatory basic state of diabetic EC and the endothelial dysfunction in diabetic disease. Therefore, this in vitro model could be used for investigating early dysfunction and environmental effects on pathological endothelium. (c) 2007 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMPAIRED GLUCOSE-TOLERANCE; SOLUBLE ADHESION MOLECULES; SERUM CONCENTRATIONS; INSULIN-RESISTANCE; E-SELECTIN; CELLS; EXPRESSION; PROTEINS; ORIGIN; MECHANISMS; endothelial dysfunction; in vitro model; diabetes mellitus; endothelial cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Dec 2020 05:52 |
| Last Modified: | 09 Dec 2020 05:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32753 |
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