Arslan, F. and Bosserhoff, A-K and Nickl-Jockschat, T. and Doerfelt, A. and Bogdahn, U. and Hau, Peter (2007) The role of versican isoforms V0/V1 in glioma migration mediated by transforming growth factor-beta 2. BRITISH JOURNAL OF CANCER, 96 (10). pp. 1560-1568. ISSN 0007-0920,
Full text not available from this repository. (Request a copy)Abstract
Versican is a large chondroitin sulphate proteoglycan produced by several tumour cell types, including high-grade glioma. The increased expression of certain versican isoforms in the extracellular matrix (ECM) plays a role in tumour cell growth, adhesion and migration. Transforming growth factor-beta 2 (TGF-beta 2) is an important modulator of glioma invasion, partially by remodeling the ECM. However, it is unknown whether it interacts with versican during malignant progression of glioma cells. Here, we analysed the effect of TGF-beta 2 on the expression of versican isoforms. The expression of versican V0/V1 was upregulated by TGF-beta 2 detected by quantitative polymerase chain reaction and immunoprecipitation, whereas V2 was not induced. Using time-lapse scratch and spheroid migration assays, we observed that the glioma migration rate is significantly increased by exogenous TGF-beta 2 and inhibited by TGF-beta 2-specific antisense oligonucleotides. Interestingly, an antibody specific for the DPEAAE region of glycosaminoglycan-beta domain of versican was able to reverse the effect of TGF-beta 2 on glioma migration in a dose-dependent manner. Taken together, we report here that TGF-beta 2 triggers the malignant phenotype of high-grade gliomas by induction of migration, and that this effect is, at least in part, mediated by versican V0/V1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-BETA; SMOOTH-MUSCLE-CELLS; HIGH-GRADE GLIOMAS; TGF-BETA; IN-VIVO; PG-M/VERSICAN; PROTEOLYTIC CLEAVAGE; INTEGRIN EXPRESSION; MALIGNANT GLIOMA; SPLICE VARIANTS; versican; TGF-beta; high-grade glioma; migration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Dec 2020 10:06 |
| Last Modified: | 09 Dec 2020 10:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32757 |
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