Schmidt, Christoph and Hoecherl, Klaus and Bucher, Michael (2007) Cytokine-mediated regulation of urea transporters during experimental endotoxemia. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 292 (5). F1479-F1489. ISSN 1931-857X, 1522-1466
Full text not available from this repository. (Request a copy)Abstract
Acute renal failure (ARF) is a frequent complication of sepsis and has a high mortality. Sepsis-induced ARF is known to be associated with significant impairment of tubular capacity. However, the pathogenesis of endotoxemic tubular dysfunction with failure of urine concentration is poorly understood. Urea plays an important role in the urinary concentrating mechanism and expression of the urea transporters UT-A1, UT-A2, UT-A3, UT-A4, and UT-B is essential for tubular urea reabsorption. The present study attempts to assess the regulation of renal urea transporters during severe inflammation in vivo. Lipopolysaccharide-(LPS)-injected mice presented with reduced glomerular filtration rate, fractional urea excretion, and inner medulla osmolality associated with a marked decrease in expression of all renal urea transporters. Similar alterations were observed after application of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, interferon (IFN)-gamma, or IL-6. LPS-induced downregulation of urea transporters was not affected in knockout mice with deficient TNF-alpha, IL-receptor-1, IFN-gamma, or IL-6. Glucocorticoid treatment inhibited LPS-induced increases of tissue TNF-alpha, IL-1 beta, IFN-gamma, or IL-6 concentration, diminished LPS- induced renal dysfunction, and attenuated the downregulation of renal urea transporters. Renal ischemia induced by renal artery clipping did not influence the expression of urea transporters. Our data demonstrate that renal urea transporters are downregulated by severe inflammation, which likely accounts for tubular dysfunction. Furthermore, they suggest that the downregulation of renal urea transporters during LPS-induced ARF is mediated by proinflammatory cytokines and is independent from renal ischemia because of sepsis-induced hypotension.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACUTE-RENAL-FAILURE; INFLAMMATORY RESPONSE SYNDROME; SEPTIC SHOCK; DOWN-REGULATION; NITRIC-OXIDE; DOUBLE-BLIND; EXPERIMENTAL PYELONEPHRITIS; CONCENTRATING ABILITY; MOLECULAR-MECHANISMS; GENE-EXPRESSION; lipopolysaccharide; glucocorticoids |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Dec 2020 10:08 |
| Last Modified: | 10 Dec 2020 10:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32776 |
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