Driemel, Oliver and Kosmehl, Hartwig and Rosenhahn, Julia and Berndt, Alexander and Reichert, Torsten E. and Zardi, Luciano and Dahse, Regine (2007) Expression analysis of extracellular matrix components in brush biopsies of oral lesions. ANTICANCER RESEARCH, 27 (3B). pp. 1565-1570. ISSN 0250-7005, 1791-7530
Full text not available from this repository. (Request a copy)Abstract
Background: Oral brush biopsies have proved to be a promising new non-invasive methodology in the diagnosis of oral lesions. The extracellular matrix (ECM) molecules gamma 2 chain of laminin-5 (L5 gamma 2), tenascin-c (Th-C) and the fibronectin isoform containing EDB (EDB-fn) are involved in matrix remodeling during malignant transformation in oral carcinoma. Materials and Methods: Expression of L5 gamma 2, Tn-C and EDB-fn was analysed in brush biopsy-obtained cells of benign inflammatory or hyperproliferative lesions and primary oral squamous cell carcinoma (OSCC) using the Roche LightCycler 2.0 System. Results and Conclusion: Oral carcinoma are detectable with mRNA resynthesis of the ECM molecules L5 gamma 2 and Tn-C in oral brush biopsies. EDB-fn mRNA was not detected - the stroma myofibroblasts are apparently a preferential source of EDB-fn and sampling by oral brush biopsy harvests epithelial cells and does not reach the cells which do express EDB-fn. The performance of gene expression analysis in brush biopsies is limited by a high RNase activity in the oral cavity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SQUAMOUS-CELL CARCINOMA; UNSPLICED TENASCIN-C; BASEMENT-MEMBRANE; MESSENGER-RNA; HUMAN BREAST; FIBRONECTIN ISOFORMS; PROTEIN DISTRIBUTION; CHRONIC INFLAMMATION; STROMAL CELLS; CANCER-CELLS; oral brush biopsy; extracellular matrix; gamma 2 chain of laminin-5; tenascin-c; EDB-fibronectin; LightCycler; ECM |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Dec 2020 10:44 |
| Last Modified: | 10 Dec 2020 10:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32784 |
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