Karczmarczyk, Aleksandra and Reiner-Rozman, Ciril and Hageneder, Simone and Dubiak-Szepietowska, Monika and Dostalek, Jakub and Feller, Karl-Heinz (2016) Fast and sensitive detection of ochratoxin A in red wine by nanoparticle-enhanced SPR. ANALYTICA CHIMICA ACTA, 937. pp. 143-150. ISSN 0003-2670, 1873-4324
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Herein, we present a fast and sensitive biosensor for detection of Ochratoxin A (OTA) in a red wine that utilizes gold nanoparticle-enhanced surface plasmon resonance (SPR). By combining an indirect competitive inhibition immunoassay and signal enhancement by secondary antibodies conjugated with gold nanoparticles (AuNPs), highly sensitive detection of low molecular weight compounds (such as OTA) was achieved. The reported biosensor allowed for OTA detection at concentrations as low as 0.75 ng mL(-1) and its limit of detection was improved by more than one order of magnitude to 0.068 ng mL(-1) by applying AuNPs as a signal enhancer. The study investigates the interplay of size of AuNPs and affinity of recognition elements affecting the efficiency of the signal amplification strategy based on AuNP. Furthermore, we observed that the presence of polyphenolic compounds in wine samples strongly interferes with the affinity binding on the surface. To overcome this limitation, a simple pre-treatment of the wine sample with the binding agent poly(vinylpyrrolidone) (PVP) was successfully applied. (C) 2016 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE-PLASMON RESONANCE; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; GOLD NANOPARTICLES; SIGNAL AMPLIFICATION; MYCOTOXINS; CLEANUP; ELISA; HPLC; QUANTIFICATION; Surface plasmon resonance; Ochratoxin A; Gold nanoparticles; Inhibition immunoassay; Signal amplification; Affinity binding |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 11:45 |
| Last Modified: | 03 Apr 2019 11:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3279 |
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