Hausmann, M. and Obermeier, F. and Paper, D. H. and Balan, K. and Dunger, N. and Menzel, K. and Falk, W. and Schoelmerich, J. and Herfarth, H. and Rogler, G. (2007) In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 148 (2). pp. 373-381. ISSN 0009-9104, 1365-2249
Full text not available from this repository. (Request a copy)Abstract
Recently we demonstrated that in inflammatory bowel disease (IBD) macrophage-oxidative burst activity is increased and NADPH oxidase mRNA is induced. The herbal phenylethanoid acteoside isolated from Plantago lanceolata L. was shown to exhibit anti-oxidative potential. Using the dextran sulphate sodium (DSS)-induced colitis model, in this study we have assessed whether systemic application of acteoside affects colitis. Colitis was induced by DSS in Balb/c mice. Treatment with acteoside (120, 600 mu g/mouse/day) was performed intraperitoneally. The colon lengths were determined. Colonic tissue was scored histologically (max. score 8) by a blinded investigator. T cells isolated from mesenteric lymph nodes (MLN) were stimulated with anti-CD3 antibody in the presence of interleukin (IL)-2 (final concentration 10 U/ml). After incubation for 24 h, IL-1 beta, IL-6, IL-12 tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma levels in supernatants were analysed by the beadlyte (R) cytokine detection system. Histological scoring of colonic tissue revealed that application of acteoside was followed by a significantly improved histological score. In acute colitis the histological score was 3.2 with acteoside versus 5.2 with phosphate-buffered saline (PBS) (P < 0.02). In chronic colitis both 120 mu g (3.3 versus 5.2) or 600 mu g acteoside (3.0 versus 5.2) significantly ameliorated colitis (both P < 0.02). Stimulated MLN from mice with chronic DSS-induced colitis treated with acteoside showed a significant down-regulation of IFN-gamma secretion (195 pg/ml with 600 mu g acteoside versus 612 pg/ml with PBS, P < 0.02). Inhibition of oxidative burst activity with acteoside reduced mucosal tissue damage in DSS colitis and could be a therapeutic alternative for IBD treatment. Further studies of this agent are warranted.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROMISING ANTINEPHRITIC AGENT; PROTEIN-KINASE-C; NITRIC-OXIDE; PHENYLPROPANOID GLYCOSIDES; OXYGEN RADICALS; BOWEL-DISEASE; NADPH OXIDASE; SIEBOLDII MIQ; EXPRESSION; ACTIVATION; acteoside; DSS; monocytes/macrophages; oxidative burst; Plantago lanceolata L. |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Dec 2020 11:01 |
| Last Modified: | 10 Dec 2020 11:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32792 |
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