Song, S. -H. and Dick, Bernhard and Penzkofer, Alfons and Hegemann, P. (2007) Photo-reduction of flavin mononucleotide to semiquinone form in LOV domain mutants of blue-light receptor phot from Chlamydomonas reinhardtii. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 87 (1). pp. 37-48. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
The photo-excitation dynamics of the mutants LOV1-C57S and LOV2-C250S of the LOV-domains of the phototropin photoreceptor phot from the green alga Chlamydomonas reinhardtii is investigated by absorption and fluorescence studies. The LOV domains fused to a maltose binding protein (MBP) are expressed in Escherichia coli. The mutants were studied under aerobic conditions in aqueous solution at pH 8. Blue-light exposure reduced the fully oxidized flavin mononucleotide, FMNox, to FMN semiquinone, FMNH-, (quantum efficiency around 1%) which further reduced to FMN hydroquinone, FMNredH2 or FMNredH- (quantum efficiency ca. 3 x 10(-5)). In the dark both reduced forms recovered back to the oxidized form on a minute timescale. Besides photoreduction, blue-light photo-excitation of the mutants resulted in photoproduct formation (efficiency in the 2 x 10(-4)-10(-3) range). Photo-reaction schemes for the mutants are discussed. (c) 2007 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EMISSION SPECTROSCOPIC CHARACTERIZATION; RHODOBACTER-SPHAEROIDES; SIGNAL-TRANSDUCTION; PHOT-LOV1 DOMAIN; AQUEOUS-SOLUTION; BINDING DOMAIN; PHOTOTROPIN; ABSORPTION; PHOTORECEPTOR; PHOTOREDUCTION; LOV domain mutants; flavin mononucleotide (FMN); flavin semiquinone; flavin hydroquinone; photo-reduction; blue-light photoreceptor; chlamydomonas reinhardtii; photo-cycle |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Molecular Spectroscopy and Photochemistry) > Prof. Dr. Bernhard Dick |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Dec 2020 16:16 |
| Last Modified: | 15 Dec 2020 16:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32900 |
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