Hashimoto, Atsushi and Tarner, Ingo H. and Bohle, Rainer M. and Gaumann, Andreas and Manetti, Mirko and Distler, Oliver and Steinmeyer, Juergen and Ulfgren, Ann-Kristin and Schulz, Andreas and Gay, Steffen and Mueller-Ladner, Ulf and Neumann, Elena (2007) Analysis of vascular gene expression in arthritic synovium by laser-mediated microdissection. ARTHRITIS AND RHEUMATISM, 56 (4). pp. 1094-1105. ISSN 0004-3591,
Full text not available from this repository. (Request a copy)Abstract
Objective. In rheumatoid arthritis (RA), formation of new blood vessels is necessary to meet the nutritional and oxygen requirements of actively proliferating synovial tissue. The aim of this study was to analyze the specific synovial vascular expression profiles of several angiogenesis-related genes as well as CD82 in RA compared with osteoarthritis (OA), using laser-mediated microdissection (LMM). Methods. LMM and subsequent real-time polymerase chain reaction were used in combination with immunohistochemical analysis for area-specific analysis of messenger RNA (mRNA) and protein expression of vascular endothelial growth factor (VEGF), VEGF receptor I (VEGFR-1), VEGFR-2, hypoxia-inducible factor 1 alpha (HIF-1 alpha), HIF-2 alpha, platelet-derived growth factor receptor alpha (PDGFR alpha), PDGFR beta, inhibitor of DNA binding/differentiation 2 (W), and CD82 in RA and OA synovial microvasculature and synovial lining. Results. Expression of Id2 mRNA was significantly lower in RA synovial vessels compared with OA synovial vessels (P = 0.0011), whereas expression of VEGFR-1 was significantly higher in RA (P = 0.0433). No differences were observed for the other parameters. At the protein level, no statistically significant differences were observed for any parameter, although Id2 levels were 2.5-fold lower in RA (P = 0.0952). However, the number of synovial blood vessels and the number of VEGFR-2-expressing blood vessels were significantly higher in RA compared with OA. Conclusion. Our results underscore the importance of area-specific gene expression analysis in studying the pathogenesis of RA and support LMM as a robust tool for this purpose. Of note, our results indicate that previously described differences between RA and OA in the expression of angiogenic molecules are attributable to higher total numbers of synovial and vascular cells expressing these molecules in RA rather than higher expression levels in the individual cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; COLLAGEN-INDUCED ARTHRITIS; LOOP-HELIX PROTEINS; RHEUMATOID-ARTHRITIS; CELL-PROLIFERATION; INDUCED ACTIVATION; TETRASPANIN CD82; BONE DESTRUCTION; HYPOXIA; ANGIOGENESIS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Dec 2020 06:37 |
| Last Modified: | 16 Dec 2020 06:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/32915 |
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