Pylypenko, Olena and Welz, Tobias and Tittel, Janine and Kollmar, Martin and Chardon, Florian and Malherbe, Gilles and Weiss, Sabine and Michel, Carina Ida Luise and Samol-Wolf, Annette and Grasskamp, Andreas Till and Hume, Alistair and Goud, Bruno and Baron, Bruno and England, Patrick and Titus, Margaret A. and Schwille, Petra and Weidemann, Thomas and Houdusse, Anne and Kerkhoff, Eugen (2016) Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. ELIFE, 5: e17523. ISSN 2050-084X,
Full text not available from this repository. (Request a copy)Abstract
There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleators and myosin V motors coordinate their specific membrane recruitment. The myosin V globular tail domain (MyoV-GTD) interacts directly with an evolutionarily conserved Spir sequence motif. We determined crystal structures of MyoVa-GTD bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form. The ternary complex architecture explains how Rab11 vesicles support coordinated F-actin nucleation and myosin force generation for vesicle transport and tethering. New insights are also provided into how myosin activation can be coupled with the generation of actin tracks. Since MyoV binds several Rab GTPases, synchronized nucleator and motor targeting could provide a common mechanism to control force generation and motility in different cellular processes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLOBULAR TAIL DOMAIN; STRUCTURAL BASIS; AMPA RECEPTORS; PROTEIN; TRANSPORT; COMPLEX; IDENTIFICATION; TRAFFICKING; ORGANIZERS; SEQUENCE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 12:06 |
| Last Modified: | 03 Apr 2019 12:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3293 |
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