Felthaus, Oliver and Prantl, Lukas and Skaff-Schwarze, Mona and Klein, Silvan and Anker, Alexandra and Ranieri, Marco and Kuehlmann, Britta (2017) Effects of different concentrations of Platelet-rich Plasma and Platelet-Poor Plasma on vitality and differentiation of autologous Adipose tissue-derived stem cells. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, 66 (1). pp. 47-55. ISSN 1386-0291, 1875-8622
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: Autologous fat grafts and adipose-derived stem cells (ASCs) can be used to treat soft tissue defects. However, the results are inconsistent and sometimes comprise tissue resorption and necrosis. This might be due to insufficient vascularization. Platelet-rich plasma (PRP) is a source of concentrated autologous platelets. The growth factors and cytokines released by platelets can facilitate angiogenesis. The simultaneous use of PRP might improve the regeneration potential of fat grafts. The optimal ratio has yet to be elucidated. A byproduct of PRP preparation is platelet-poor plasma (PPP). OBJECTIVE: In this study we investigated the influence of different concentrations of PRP on the vitality and differentiation of ASCs. METHODS: We processed whole blood with the Arthrex Angel centrifuge and isolated ASCs from the same donor. We tested the effects of different PRP and PPP concentrations on the vitality using resazurin assays and the differentiation of ASCs using oil-red staining. RESULTS: Both cell vitality and adipogenic differentiation increase to a concentration of 10% to 20% PRP. With a PRP concentration of 30% cell vitality and differentiation decrease. CONCLUSIONS: Both PRP and PPP can be used to expand ASCs without xenogeneic additives in cell culture. A PRP concentration above 20% has inhibitory effects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VIVO BONE-FORMATION; ASSISTED LIPOTRANSFER; FAT TRANSPLANTATION; GROWTH-FACTORS; BASIC SCIENCE; BROWN; TERM; PROLIFERATION; LIPOSUCTION; EXPRESSION; Adipose tissue derived stem cells; platelet rich plasma; tissue engineering |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 12:57 |
| Last Modified: | 25 Feb 2019 11:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33 |
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