Denk, Alexandra E. and Bettstetter, Marcus and Wild, Peter J. and Hoek, Keith and Bataille, Frauke and Dietmaier, Wolfgang and Bosserhoff, Anja K. (2007) Loss of maspin expression invasive potential in malig contributes to a more nant melanoma. PIGMENT CELL RESEARCH, 20 (2). pp. 112-119. ISSN 0893-5785,
Full text not available from this repository. (Request a copy)Abstract
Deregulation of protease expression and activity is known to play an important role in tumour progression of malignant melanoma. The serpin maspin, a tumour suppressor in breast and prostate cancer was described as an inhibitor of cell migration and inducer of cell adhesion between the basement membrane and extracellular matrix resulting in inhibition of tumour metastasis. In contrast, overexpression of maspin is correlated with poor prognosis in other cancers. However, little is known about expression, regulation and function of maspin in malignant melanoma. In this study, we found loss of maspin expression in malignant melanoma cells compared with normal human epidermal melanocytes, which was analysed by quantitative real-time PCR, Western blot analysis, immunohistochemistry and microarray. For functional studies, melanoma cell clones stably transfected with a maspin expression vector were tested for changes in proliferation, migration and invasion. Although we could not see differences in proliferation and migration, we detected strongly reduced invasive capacity in the melanoma cell clones in which maspin is re-expressed compared with control. Reduced invasive potential was also detected in three different melanoma cell lines transiently transfected with a maspin expression vector. Furthermore, exogenously added maspin alone was sufficient to reduce invasion in Mellm significantly, indicating that maspin directly inhibits invasion on the cell surface. In summary, we believe that maspin is a tumour suppressor in malignant melanoma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-SUPPRESSOR GENE; INHIBITORY-ACTIVITY MIA; HUMAN BREAST-CANCER; MATRIX METALLOPROTEINASES; CELL INVASION; LUNG-CANCER; METASTASIS; SERPIN; METHYLATION; CARCINOMA; maspin; serpin; melanoma; primary melanocytes; MMP; uPA; qRT-PCR |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2020 09:19 |
| Last Modified: | 21 Dec 2020 09:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33005 |
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