Elena Gomez, Carmen and Luis Najera, Jose and Jimenez, Victoria and Bieler, Kurt and Wild, Jens and Kostic, Linda and Heidari, Shirin and Chen, Margaret and Frachette, Marie-Joelle and Pantaleo, Giuseppe and Wolf, Hans and Liljestrom, Peter and Wagner, Ralf and Esteban, Mariano (2007) Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade C. VACCINE, 25 (11). pp. 1969-1992. ISSN 0264-410X, 1873-2518
Full text not available from this repository. (Request a copy)Abstract
Recombinants based on the attenuated vaccinia virus strains MVA and NYVAC are considered candidate vectors against different human diseases. In this study we have generated and characterized in BALB/c and in transgenic HHD mice the immunogenicity of two attenuated poxvirus vectors expressing in a single locus (TK) the codon optimized HIV-1 genes encoding gp120 and Gag-Pol-Nef (GPN) polyprotein of clade C (referred as MVA-C and NYVAC-Q. In HHD mice primed with either MVA-C or NYVAC-C, or primed with DNA-C and boosted with the poxvirus vectors, the splenic T cell responses against clade C peptides spanning gp120/GPN was broad and mainly directed against Gag-1, Env-1 and Env-2 peptide pools. In BALB/c mice immunized with the homologous or the heterologous combination of poxvirus vectors or with Semliki forest virus (SFV) vectors expressing gp120/GPN, the immune response was also broad but the most immunogenic peptides were Env-1, GPN-1 and GPN-2. Differences in the magnitude of the cellular immune responses were observed between the poxvirus vectors depending on the protocol used. The specific cellular immune response triggered by the poxvirus vectors was Th1 type. The cellular response against the vectors was higher for NYVAC than for MVA in both HHD and BALB/c mice, but differences in viral antigen recognition between the vectors was observed in sera from the poxvirus-immunized animals. These results demonstrate the immunogenic potential of MVA-C and NYVAC-C as novel vaccine candidates against clade C of HIV-1. (c) 2006 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMMUNODEFICIENCY-VIRUS-INFECTION; CYTOTOXIC T-LYMPHOCYTES; SEMLIKI-FOREST-VIRUS; FULL-LENGTH CLONES; TYPE-1 SUBTYPE C; IMMUNE-RESPONSES; CELL RESPONSES; AIDS VACCINE; IN-VIVO; CD8(+) LYMPHOCYTES; HIV/AIDS; clade C vaccine; poxvirus vectors; MVA; NYVAC; DNA vectors; SFV vectors; immune response; mouse models |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2020 07:42 |
| Last Modified: | 22 Dec 2020 07:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33164 |
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