Yegorova, Alla V. and Scripinets, Yulia V. and Duerkop, Axel and Karasyov, Alexander A. and Antonovich, Valery P. and Wolfbeis, Otto S. (2007) Sensitive luminescent determination of DNA using the terbium(III)-difloxacin complex. ANALYTICA CHIMICA ACTA, 584 (2). pp. 260-267. ISSN 0003-2670,
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The interaction of the terbium-difloxacin complex (Tb-DFX) with DNA has been examined by using UV-vis absorption and luminescence spectroscopy. The Tb-DFX complex shows an up to 85-fold enhancement of luminescence intensity upon titration with DNA. The long decay times allow additional detection schemes like time-resolved measurements in microplate readers to enhance sensitivity by off-gating short-lived background luminescence. Optimal conditions are found at equimolar concentrations of Tb3+ and DFX (0.1 or 1 mu M) at pH 7.4. Under these conditions, the luminescence intensity is linearly dependent on the concentration of ds-DNAs and ss-DNA between 1-1500 ng mL(-1) and 4.5-270 ng mL(-1), respectively. The detection limit is 0.5 ng mL(-1) for ds-DNAs and 2 ng mL(-1) for ss-DNA. The mechanism for the luminescence enhancement was also studied. (c) 2006 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DOUBLE-STRANDED DNA; INDUCED FLUORESCENCE DETECTION; NUCLEIC-ACIDS; CAPILLARY-ELECTROPHORESIS; BINDING; NORFLOXACIN; PROBES; ASSAY; ENHANCEMENT; DYES; UV-vis spectrometry; luminescence; terbium; quinolones; difloxacin; DNA |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2020 09:55 |
| Last Modified: | 22 Dec 2020 09:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33176 |
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