Yacoub-George, Erwin and Hell, Waltraud and Meixner, Leonhard and Wenninger, Franz and Bock, Karlheinz and Lindner, Petra and Wolf, Hans and Kloth, Tanja and Feller, Klaus A. (2007) Automated 10-channel capillary chip immunodetector for biological agents detection. BIOSENSORS & BIOELECTRONICS, 22 (7). pp. 1368-1375. ISSN 0956-5663, 1873-4235
Full text not available from this repository. (Request a copy)Abstract
The automated 10-channel capillary chip immunodetector (10K-IDWG) is a prototype, which has been developed for automatically operated biological agents (BA) point detection. The current technology uses a chemiluminescence capillary immunoassay (EIA) technique in combination with integrated microfluidics and allows the highly sensitive and rapid detection and preliminary identification of multiple BA in aqueous solutions in the laboratory. The chemiluminescence capillary EIA are performed within a disposable capillary chip containing 10 fused-silica capillaries arranged in parallel coated with selected capture antibodies. A multianode-photomultiplier array is used to detect chemiluminescence intensity in each capillary. Reservoirs for reagents and buffers and a waste disposal reservoir are integrated. This paper describes the technology of the 10K-IDWG and its evaluation with three different BA, the toxin staphylococcal enterotoxin B (SEB), the bacterial analyte Escherichia coli (E. coli) O157:H7 as a model for bacterial pathogens, and the bacteriophage M13 as a model for virus pathogens. The 10K-IDWG is able to detect the above mentioned three BA in an aqueous sample within 29 min (single analyte-detection and multiplexing). Limits of detection (LOD) are 0.1 ng/ml for SEB, 10(4) cfu/ml for E. coli O 157:1-17, and 5 x 10(5) pfu/ml for M13. Cross reactivities between the three assays were not observed. (c) 2006 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARRAY BIOSENSOR; MULTICHANNEL IMMUNOSENSOR; SYSTEM; IMMUNOASSAY; ANTIBODY; ASSAYS; biosensor; chemiluminescence; capillary enzyme immunoassay; multiplex detection; biological agent; microfluidics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2020 10:12 |
| Last Modified: | 22 Dec 2020 10:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33181 |
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