Bauer, Johann and Kopp, Sascha and Schlagberger, Elisabeth Maria and Grosse, Jirka and Sahana, Jayashree and Riwaldt, Stefan and Wehland, Markus and Luetzenberg, Ronald and Infanger, Manfred and Grimm, Daniela (2017) Proteome Analysis of Human Follicular Thyroid Cancer Cells Exposed to the Random Positioning Machine. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18 (3): 546. ISSN 1422-0067,
Full text not available from this repository. (Request a copy)Abstract
Several years ago, we detected the formation of multicellular spheroids in experiments with human thyroid cancer cells cultured on the Random Positioning Machine (RPM), a ground-based model to simulate microgravity by continuously changing the orientation of samples. Since then, we have studied cellular mechanisms triggering the cells to leave a monolayer and aggregate to spheroids. Our work focused on spheroid-related changes in gene expression patterns, in protein concentrations, and in factors secreted to the culture supernatant during the period when growth is altered. We detected that factors inducing angiogenesis, the composition of integrins, the density of the cell monolayer exposed to microgravity, the enhanced production of caveolin-1, and the nuclear factor kappa B p65 could play a role during spheroid formation in thyroid cancer cells. In this study, we performed a deep proteome analysis on FTC-133 thyroid cancer cells cultured under conditions designed to encourage or discourage spheroid formation. The experiments revealed more than 5900 proteins. Their evaluation confirmed and explained the observations mentioned above. In addition, we learned that FTC-133 cells growing in monolayers or in spheroids after RPM-exposure incorporate vinculin, paxillin, focal adhesion kinase 1, and adenine diphosphate (ADP)-ribosylation factor 6 in different ways into the focal adhesion complex.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOCAL ADHESION KINASE; SIMULATED MICROGRAVITY CONDITIONS; GTPASE-ACTIVATING PROTEIN; SPHEROID FORMATION; 3-DIMENSIONAL GROWTH; CARCINOMA CELLS; IDENTIFICATION; MIGRATION; PAXILLIN; ASAP1; cellular compartments; mass spectrometry; proteomics; pathway analysis; random positioning machine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 26 Feb 2019 12:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/332 |
Actions (login required)
 |
View Item |
