Ousingsawat, Jiraporn and Spitzner, Melanie and Puntheeranurak, Supaporn and Terracciano, Luigi and Tornillo, Luigi and Bubendorf, Lukas and Kunzelmann, Karl and Schreiber, Rainer (2007) Expression of voltage-gated potassium channels in human and mouse colonic carcinoma. CLINICAL CANCER RESEARCH, 13 (3). pp. 824-831. ISSN 1078-0432, 1557-3265
Full text not available from this repository. (Request a copy)Abstract
Purpose: Voltage-gated Kv potassium channels, like ether a go-go (EAG) channels, have been recognized for their oncogenic potential in breast cancer and other malignant tumors. Experimental Design: We examined the molecular and functional expression of Kv channels in human colonic cancers and colon of mice treated with the chemical carcinogens dimethylhydrazine and N-methyl-N-nitrosourea. The data were compared with results from control mice and animals with chemically induced DSS colitis. Results: Electrogenic salt transport by amiloride-sensitive Na+ channels and cyclic AMP activated cystic fibrosis transmembrane conductance regulator Cl- channels were attenuated during tumor development and colitis, whereas Ca-21-dependent transport remained unchanged. Kv channels, in particular Eag-1, were enhanced during carcinogenesis. Multiplex reverse transcription-PCR showed increased mRNA expression for Kv1.3, Kv1.5, Kv3.1, and members of the Eag channel family, after dimethylhydrazine and N-methyl-N-nitrosourea treatment. Eag-1 protein was detected in the malignant mouse colon and human colonic cancers. Genomic amplification of Eag-1 was found in 3.4% of all human colorectal adenocarcinoma and was an independent marker of adverse prognosis. Conclusions: The study predicts an oncogenic role of Kv and Eag channels for the development of colonic cancer. These channels may represent an important target for a novel pharmacotherapy of colonic cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHYL-N-NITROSOUREA; CELL-PROLIFERATION; ION CHANNELS; K+ CHANNELS; CANCER; CARCINOGENESIS; INDUCTION; MICE; MARKERS; ETHER; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2020 10:11 |
| Last Modified: | 22 Dec 2020 10:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33203 |
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