Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene

Reischl, U. and Tuohy, M. J. and Hall, G. S. and Procop, G. W. and Lehn, N. and Linde, H. (2007) Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 26 (2). pp. 131-135. ISSN 0934-9723,

Full text not available from this repository. (Request a copy)

Abstract

In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.

Item Type: Article
Uncontrolled Keywords: METHICILLIN-RESISTANT; FLUORESCENCE PCR; COMMUNITY; INFECTIONS; STRAINS; LEUCOCIDIN; PNEUMONIA; MRSA; ASSOCIATION; PREVALENCE;
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Depositing User: Dr. Gernot Deinzer
Date Deposited: 22 Dec 2020 10:20
Last Modified: 22 Dec 2020 10:20
URI: https://pred.uni-regensburg.de/id/eprint/33208

Actions (login required)

View Item View Item