Schaeffler, Andreas and Weigert, Johanna and Neumeier, Markus and Schoelmerich, Juergen and Buechler, Christa (2007) Regulation and function of collagenous repeat containing sequence of 26-kDa protein gene product "Cartonectin". OBESITY, 15 (2). pp. 303-313. ISSN 1930-7381,
Full text not available from this repository. (Request a copy)Abstract
Objective: Collagenous repeat containing sequence of 26kDa protein (CORS-26) was identified as a new gene transcript expressed in cartilage with unknown function. It was the aim of this study to investigate expression, regulation, and function of CORS-26 in adipocytes. Research Methods and Procedures: CORS-26 mRNA and protein expression was studied by reverse transcriptase-polymerase chain reaction, Western blot analysis, and quantitative real-time polymerase chain reaction. Transcriptional regulation was studied by electrophoretic mobility shift assay and luciferase reporter gene assay. The adipocytic secretion of adiponectin and resistin was measured by enzyme-linked immunosorbent assay. Results: CORS-26 mRNA is absent in 3T3-L1 preadipocytes and adipocytes after 48 hours of differentiation. CORS-26 mRNA was induced from Day 4 to Day 9 of adipocyte differentiation. CORS-26 protein was induced in mature adipocytes. Peroxisome proliferator-activated receptor (PPAR) gamma (but not PPAR alpha) in nuclear extracts prepared from adipocytes was shown to bind specifically to a putative peroxisome proliferator response element-one-half-site located at -641/-596 bp. Increasing doses of the ligands troglitazone (1, 10, 20 mu M) and fenofibrate (50, 100, 200 mu M) but not 15-deoxy-prostaglandin (J(2)) (0.5, 1.0, 2.5 mu M) resulted in a significant reduction of both promoter activity and the amount of mRNA expression. Recombinant CORS-26 significantly stimulated the adipocytic secretion of adiponectin and resistin in a dose-dependent manner. Discussion: The mRNA and protein expression profile puts CORS-26 in the adipocytokine family. Cartonectin is negatively regulated by exogenous, but not endogenous, PPAR gamma ligands. Because CORS-26 up-regulates adipokine secretion, it might be involved in metabolic and immunologic pathways.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED RECEPTOR-GAMMA; ADIPOSE CELL LINE; COA OXIDASE GENE; TRANSCRIPTIONAL REGULATION; ADIPOCYTE DIFFERENTIATION; CHROMOSOMAL LOCALIZATION; GENOMIC ORGANIZATION; 3T3-L1 ADIPOCYTES; RESPONSE ELEMENT; LEPTIN PROMOTER; collagenous repeat containing sequence of 26-kDa protein; adipocyte; peroxisome proliferator-activated; receptor gamma; adiponectin; resistin |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2020 15:06 |
| Last Modified: | 22 Dec 2020 15:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33255 |
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