Babinger, Patrick and Voelkl, R. and Cakstina, I. and Maftei, A. and Schmitt, R. (2007) Maintenance DNA methyltransferase (Met1) and silencing of CpG-methylated foreign DNA in Volvox carteri. PLANT MOLECULAR BIOLOGY, 63 (3). pp. 325-336. ISSN 0167-4412,
Full text not available from this repository. (Request a copy)Abstract
DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are taking place. It encodes a 238 kDa protein containing eight C-terminal activity domains typical of M-MTases, plus upstream DNA-binding domains including the ProDom domain PD003757, which experimental analyses in animal systems have indicated is required for targeting the enzyme to DNA-replication foci. Several insertions of unknown function make Volvox Met1 the largest known member of the Met1/Dnmt1 family. Here we also show that several endogenous transposon families are CpG-methyated in Volvox, which we think causes them to be inactive. This view is supported by the observation that an in vitro CpG-methylated gene introduced into Volvox was maintained in the methylated and silent state over > 100 generations. Thus, we believe that Met1 recognizes and perpetuates the in vitro methylation signal, and that the silencing machinery is then able to transduce such a methylation-only signal into a stable heterochromatic (and silent) state.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GERM-SOMA DIFFERENTIATION; ASEXUAL LIFE-CYCLE; EXPRESSION; PROTEIN; GENE; REPLICATION; TRANSPOSONS; CHROMATIN; DIVISION; ENCODES; CpG methylation; bisulfite genomic sequencing; transgene silencing; transposon silencing; DNA methyltransferase; Volvox carteri |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2020 15:35 |
| Last Modified: | 22 Dec 2020 15:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33261 |
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