Heber, Sabine and Denk, Lucia and Hu, Kanghong and Minuth, Will W. (2007) Modulating the development of renal tubules growing in serum-free culture medium at an artificial interstitium. TISSUE ENGINEERING, 13 (2). pp. 281-292. ISSN 1076-3279,
Full text not available from this repository. (Request a copy)Abstract
Little information on the structural growth of renal tubules is available. A major problem is the technical limitation of culturing intact differentiated tubules over prolonged periods of time. Consequently, we developed an advanced culture method to follow tubule development. Isolated tissue containing renal progenitor cells was placed in a perfusion culture container at the interphase of an artificial polyester interstitium. Iscove's modified Dulbecco's medium without serum or protein supplementation was used for culture, and the culture period was 13 days. Tissue growth was not supported by addition of extracellular matrix proteins. The development of tubules was registered on cryosections labeled with soybean agglutinin (SBA) and tissue-specific antibodies. Multiple SBA-labeled tubules were found when aldosterone was added to the culture medium. In contrast, culture without aldosterone supplementation displayed completely disintegrated tissue. The development of tubules depended on the applied aldosterone concentration. The use of 1 x 10(-6) M and 1 x 10(-7) M aldosterone produced numerous tubules, while application of 1 x 10(-8) M to 1 x 10(-10) M led to a continuous decrease and finally a loss of tubule formation. The development of labeled tubules in aldosterone-treated specimens took an unexpectedly long period of at least 8 days. The morphogenic effect of aldosterone appeared to be mineralocorticoid hormone-specific since spironolactone and canrenoate abolished the development. Finally, dexamethasone induced widely distributed cell clusters instead of tubules.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLLECTING DUCT; STEM-CELLS; DEVELOPING KIDNEY; NEPHRON SEGMENTS; EMBRYONIC KIDNEY; RABBIT KIDNEY; ORGAN-CULTURE; URETERAL BUD; MICRO-FIBERS; DIFFERENTIATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Dec 2020 06:28 |
| Last Modified: | 23 Dec 2020 06:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33276 |
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