Grossmann, Guido and Opekarova, Miroslava and Malinsky, Jan and Weig-Meckl, Ina and Tanner, Widmar (2007) Membrane potential governs lateral segregation of plasma membrane proteins and lipids in yeast. EMBO JOURNAL, 26 (1). pp. 1-8. ISSN 0261-4189,
Full text not available from this repository. (Request a copy)Abstract
The plasma membrane potential is mainly considered as the driving force for ion and nutrient translocation. Using the yeast Saccharomyces cerevisiae as a model organism, we have discovered a novel role of the membrane potential in the organization of the plasma membrane. Within the yeast plasma membrane, two non-overlapping subcompartments can be visualized. The first one, represented by a network-like structure, is occupied by the proton ATPase, Pma1, and the second one, forming 300-nm patches, houses a number of proton symporters (Can1, Fur4, Tat2 and HUP1) and Sur7, a component of the recently described eisosomes. Evidence is presented that sterols, the main lipid constituent of the plasma membrane, also accumulate within the patchy compartment. It is documented that this compartmentation is highly dependent on the energization of the membrane. Plasma membrane depolarization causes reversible dispersion of the H+-symporters, not however of the Sur7 protein. Mitochondrial mutants, affected in plasma membrane energization, show a significantly lower degree of membrane protein segregation. In accordance with these observations, depolarized membranes also considerably change their physical properties (detergent sensitivity).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; CELL-SURFACE; CHLORELLA-VULGARIS; DOMAIN FORMATION; URACIL PERMEASE; H+-ATPASE; RAFTS; GENES; EXPRESSION; COMPARTMENTATION; H+-symporters; lipid rafts; membrane compartmentation; susceptibility against detergents |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Dec 2020 08:54 |
| Last Modified: | 23 Dec 2020 08:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33299 |
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