Sternfeld, Thomas and Schmid, Mathias and Tischleder, Annette and Mudra, Susanne and Schlamp, Angelika and Kost, Bernd P. and Gruber, Rudolf and Youle, Mike and Bogner, Johannes R. and Goebel, Frank-Detlef (2007) The influence of HIV infection and antiretroviral therapy on the mitochondrial membrane potential of peripheral mononuclear cells. ANTIVIRAL THERAPY, 12 (5). pp. 769-778. ISSN 1359-6535,
Full text not available from this repository. (Request a copy)Abstract
Objectives: Clinical disorders occurring in HIV-infected patients on antiretroviral therapy (ART) have been linked to mitochondrial dysfunction, for example, lactic acidosis and lipodystrophy. Mitochondrial membrane potential (Delta Psi(m)) is the most direct measure of the state of energization of the mitochondria. We analysed Delta Psi(m) of peripheral blood mononuclear cells (PBMCs) in HIV-negative, healthy subjects (n = 8), HIV-infected, treatment-naive patients (n = 30), and HIV-infected patients on ART (n = 58). The influence of ART was analysed in six patients who started their first regimen. Methods: The Delta Psi(m) of PBMC was measured by flow cytometry using the dye JC-1. Results: The Delta Psi(m) was significantly lower in HIV-infected patients than in HIV-negative controls. This difference was detected in both treated (P = 0.0001) and untreated patients (P = 0.001). The Delta Psi m of PBMCs was highly correlated with CD4(+) T-cell count in therapy-naive patients (P = 0.002, r = 0.546) and in treated patients (P = 0.028, r = 0.288). The Delta Psi(m) increased significantly in therapy-naive patients after starting ART (P = 0.001). Patients with lipoatrophy had significantly lower Delta Psi(m) than patients without lipodystrophy or with lipohypertrophy (P = 0.023). Conclusions: In HIV-infected persons Delta Psi(m) is significantly reduced. Patients with lipoatrophy have significantly reduced Delta Psi(m). This is the first study showing that the Delta Psi(m) of PBMCs is highly correlated with CD4(+) T-cell count in HIV infection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REVERSE-TRANSCRIPTASE INHIBITORS; BLOOD-CELLS; DNA CONTENT; INTACT-CELLS; TOXICITY; NUCLEOSIDE; LIPODYSTROPHY; LYMPHOCYTES; APOPTOSIS; DEPLETION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Jan 2021 08:34 |
| Last Modified: | 11 Jan 2021 08:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33334 |
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