Puntheeranurak, Supaporn and Schreiber, Rainer and Spitzner, Melanie and Ousingsawat, Jiraporn and Krishnamra, Nateetip and Kunzelmann, Karl (2007) Control of ion transport in mouse proximal and distal colon by prolactin. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 19 (1-4). pp. 77-88. ISSN 1015-8987, 1421-9778
Full text not available from this repository. (Request a copy)Abstract
The lactogenic hormone prolactin (PRL) has been known to affect Ca2+ and electrolyte transport in the intestinal epithelium. In the present study we analyzed ion transport in mouse proximal and distal colon, and acute changes induced by PRL. In the proximal colon, carbachol activated a Ca2+ dependent Cl- secretion that was sensitive to DIDS and NFA. In the distal colon, both ATP and carbachol activated K+ secretion. Ca2+ - activated KCl transport in proximal and distal colon was inhibited by PRL (200ng/ml), while amiloride sensitive Na+ absorption and cAMP induced Cl- secretion remained unaffected. Luminal large conductance Ca2+-activated K+ (BK) channels were largely responsible for Ca2+-activated K+ secretion in the distal colon, and basolateral BK channels supported Ca2+-activated Cl- secretion in the proximal colon. Ca2+ chelating by BAPTA-AM attenuated effects of carbachol and abolished effects of PRL. Both inhibition of PI3 kinase with wortmannin and blockage of MAP kinases with SB 203580 or U 0126, interfered with the acute inhibitory effect of PRL on ion transport, while blocking of Jak/Stat kinases with AG 490 was without effects. PRL attenuated the increase in intracellular Ca2+ that was caused by stimulation of isolated colonic crypts with carbachol. Thus PRL inhibits Ca2+ dependent Cl- and K+ secretion by interfering with intracellular Ca2+ signaling and probably by activating PI3 kinase and MAP kinase pathways. Copyright (c) 2007 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED PROTEIN-KINASE; VIVO PERFUSION TECHNIQUE; GROWTH-FACTOR RECEPTOR; PHOSPHATIDYLINOSITOL 3-KINASE; POTASSIUM CHANNELS; EPITHELIAL-CELLS; SODIUM-CHLORIDE; BK CHANNELS; CALCIUM; CONDUCTANCE; prolactin; BK channels; CFTR; EnaC; Ca2+-activated Cl channels; epithelial transport; secretion; colon; ion channels |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Jan 2021 10:23 |
| Last Modified: | 11 Jan 2021 10:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33354 |
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