Spitzner, Melanie and Ousingsawat, Jiraporn and Scheidt, Kerstin and Kunzelmann, Karl and Schreiber, Rainer (2007) Voltage-gated K+ channels support proliferation of colonic carcinoma cells. FASEB JOURNAL, 21 (1). pp. 35-44. ISSN 0892-6638, 1530-6860
Full text not available from this repository. (Request a copy)Abstract
Plasma membrane potassium ( K+) channels are required for cell proliferation. Evidence is growing that K+ channels play a central role in the development and growth of human cancer. Here we examine the contribution and the mechanism by which K+ channels control proliferation of T-84 human colonic carcinoma cells. Numerous K+ channels are expressed in T-84 cells, but only voltage-gated K+ ( Kv) channels influenced proliferation. A number of Kv channel inhibitors reduced DNA synthesis and cell number, without exerting apoptotic or toxic effects. Expression of several Kv channels, such as EagI, Kv 3.4 and Kv 1.5, was detected in patch clamp experiments and in fluorescence-based assays using a voltage sensitive dye. The contribution of EagI channels to proliferation was confirmed by siRNA, which abolished EagI activity and inhibited cell growth. Inhibition of Kv channels did not interfere with the ability of T-84 cells to regulate their cell vol, but it restricted intracellular pH regulation. In addition, inhibitors of Kv channels, as well as siRNA for EagI, attenuated intracellular Ca2+ signaling. The data suggest that Kv channels control proliferation of colonic cancer cells by affecting intracellular pH and Ca2+ signaling.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POTASSIUM-ION CHANNELS; RNA INTERFERENCE; INTRACELLULAR PH; PROSTATE-CANCER; MESSENGER-RNA; CA2+ INFLUX; HT29 CELLS; EXPRESSION; PROGRESSION; PROTEIN; Eag; T-84; intracellular Ca2+; growth; Ussing chamber; patch clamp; intracellular pH; ion channels cancer |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Jan 2021 07:41 |
| Last Modified: | 12 Jan 2021 07:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33415 |
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