Lippert, E. and Falk, W. and Bataille, F. and Kaehne, T. and Naumann, M. and Goeke, M. and Herfarth, H. and Schoelmerich, J. and Rogler, Gerhard (2007) Soluble galectin-3 is a strong, colonic epithelial-cell-derived, lamina propria fibroblast-stimulating factor. GUT, 56 (1). pp. 43-51. ISSN 0017-5749,
Full text not available from this repository. (Request a copy)Abstract
Background: Colonic lamina propria fibroblasts (CLPFs) play an important role in the pathogenesis of fibrosis and strictures in Crohn's disease. Aim: To identify colonic epithelial cell (CEC)-derived factors that activate CLPFs. Methods: Primary human CECs and CLPFs were isolated from control mucosa and interleukin 8 (IL8) of CLPF cultures was quantified by ELISA. Activation of nuclear factor kappa B (NF-kappa B) was shown, and translocation of NF-kappa B was inhibited by a dominant-negative I kappa B-expressing adenovirus. The major CLPF-activating and IL8 inducing protein was purified using fast-performance liquid chromatography (HiPrep 16/60 Sephacryl S-200 High Resolution Column) and sodium dodecyl sulphate gel electrophoresis. Results: A considerable increase in IL8 secretion by CLPFs cultured in CEC-conditioned media compared with that in unconditioned media (155.00 (10.00) pg/mu g v 1.434 (0.695) pg/mu g) was found. The effect of CEC-conditioned media on CLPF IL8 secretion was NF-kappa B dependent. A protein or DNA array confirmed the involvement of NF-kappa B and activator protein-1. Purification of a candidate band isolated with the use of sodium dodecyl sulphate-polyacrylamide gel electrophoresis and subsequent sequencing showed soluble galectin-3 to be a strong CLPF-activating factor. Depletion of galectin-3 from conditioned media by immunoprecipitation abolished the CLPF stimulatory effect. Conclusions: Using a classical biochemical approach, soluble galectin-3 was identified as a strong activator of CLPFs produced by CEC. Galectin-3 induced NF-kappa B activation and IL8 secretion in these cells and may be a target for future therapeutic approaches to reduce or avoid stricture formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; CROHNS-DISEASE; GROWTH-FACTOR; INFLAMMATORY RESPONSES; CYTOKINE PRODUCTION; WOUND CONTRACTION; BINDING PROTEIN; MESSENGER-RNA; EXPRESSION; ACTIVATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Jan 2021 07:58 |
| Last Modified: | 12 Jan 2021 07:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33419 |
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