Amslinger, Sabine and Hecht, Stefan and Rohdich, Felix and Eisenreich, Wolfgang and Adam, Petra and Bacher, Adelbert and Bauer, Stefan (2007) Stimulation of V gamma 9/V delta 2 T-lymphocyte proliferation by the isoprenoid precursor, (E)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate. IMMUNOBIOLOGY, 212 (1). pp. 47-55. ISSN 0171-2985,
Full text not available from this repository. (Request a copy)Abstract
(E)-1-Hydroxy-2-methyl-but-2-enyl 4-diphosphate, a recently discovered intermediate in the deoxyxylulose phosphate pathway of isoprenoid biosynthesis, has been shown to act as a potent immunomodulator. In cultures of human peripheral blood mononuclear cells from eight non-related donors, the compound stimulated the proliferation of V gamma 9/V delta 2 T lymphocytes with a median EC50 of 70pM when 10U/ml of IL-2 was used as costimulant. Isopentenyl diphosphate (IPP), dimethylallyl diphosphate (DMAPP) and some structural analogs of (E)-1-hydroxy-2-methyl-but2-enyl 4-diphosphate also stimulated V gamma 9/V delta 2 T-cell proliferation, albeit at much higher concentrations. The VT9/VJ2 T-cell proliferation is highly dependent on the seeding density used in culture. All phosphoantigens tested elicited the proliferation of two T-lymphocyte populations with different apparent ratios between the expression level of V delta 2 and V gamma 9 chains. (c) 2006 Elsevier GmbH. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEOXYXYLULOSE PHOSPHATE-PATHWAY; CELL-RECEPTOR-GAMMA; NONPEPTIDE ANTIGENS; NONMEVALONATE PATHWAY; BIOSYNTHETIC-PATHWAY; MEVALONATE PATHWAY; ESCHERICHIA-COLI; GENOME SEQUENCE; DELTA CELLS; PYROPHOSPHATE; deoxyxylulose phosphate; gamma delta T-cell activation; 1-hydroxy-2-methyl-but-2-enyl 4-diphosphate; isoprenoid biosynthesis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Jan 2021 09:28 |
| Last Modified: | 12 Jan 2021 09:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33428 |
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