Li, Junwei and Laengst, Gernot and Grummt, Ingrid (2006) NoRC-dependent nucleosome positioning silences rRNA genes. EMBO JOURNAL, 25 (24). pp. 5735-5741. ISSN 0261-4189, 1460-2075
Full text not available from this repository. (Request a copy)Abstract
Previous studies have established that the Snf2h-containing chromatin remodeling complex NoRC mediates epigenetic silencing of a subset of rRNA genes ( rDNA) by recruiting enzymatic activities that modify histones and methylate DNA. Here we have analyzed nucleosome positions at the murine rDNA promoter and show that active and silent rDNA copies are characterized not only by specific epigenetic marks but also by differently positioned nucleosomes. At active genes the promoter-bound nucleosome covers nucleotides from -157 to -2, whereas at silent genes the nucleosome is positioned 25 nucleotides further downstream. We provide evidence that NoRC is the molecular machine that shifts the promoter-bound nucleosome downstream of the transcription start site into a translational position that is unfavorable for transcription complex formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REMODELING COMPLEX NORC; POLYMERASE-I TRANSCRIPTION; CHROMATIN STRUCTURE; REPRESSION; ROLES; ISWI; ORGANIZATION; REPLICATION; METHYLATION; PROMOTER; differentiation; NoRC; nucleosome positioning; RNA polymerase I; TIP5 |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jan 2021 12:37 |
| Last Modified: | 15 Jan 2021 12:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33657 |
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