Waddell, Nic and Jonnalagadda, Jyoti and Marsh, Anna and Grist, Scott and Jenkins, Mark and Hobson, Karen and Taylor, Malcolm and Lindeman, Geoff J. and Tavtigian, Sean V. and Suthers, Graeme and Goldgar, David and Oefner, Peter J. and Taylor, Darrin and Grimmond, Sean and Khanna, Kum Kum and Chenevix-Trench, Georgia (2006) Characterization of the breast cancer associated ATM 7271T > G (V2424G) mutation by gene expression profiling. GENES CHROMOSOMES & CANCER, 45 (12). pp. 1169-1181. ISSN 1045-2257,
Full text not available from this repository. (Request a copy)Abstract
Mutations in ATM are responsible for the autosomal recessive disorder ataxia telangiectasia. Heterozygous mutations in ATM have been associated with an elevated risk of breast cancer. We previously reported one breast cancer family in which ATM 7271 T > G (V2424G) segregated with disease, and apparently acted in a dominant negative manner. We now report the screening of 782 multiple-case breast cancer families that identified two additional index cases with ATM 7271T > G. Phylogenetic sequence analysis showed that V2424 is a highly conserved residue, and that the 2424G variant is likely to interfere with function. To elucidate the consequences of this mutation, we expression profiled wild-type, heterozygous, and homozygous lymphoblastoid cell lines (LCLs) from Scottish and Australian families using an oligonucleotide microarray. Cluster analysis revealed 77 genes that were differentially expressed in homozygous and heterozygous V2424G cells (compared to wild-type) and I I genes differentially expressed in the homozygous cells. We also evaluated the profiles of LCLs after exposure to ionizing radiation (IR) and identified 77 genes that were differentially expressed in wild-type cells, but not in homozygous or heterozygous V2424G cells. We validated the expression differences by RT-PCR in additional heterozygous V2424G LCLs from another breast cancer family. We found no consistent cytotoxicity or abrogation of ATM kinase activity after IR in seven heterozygous V2424G LCLs, compared to wild-type LCLs, but did find an increase in the number of chromosomal aberrations. These data suggest that the V2424G missense mutation acts largely as a dominant negative in terms of the associated expression profiles. This article contains Supplementary Material available at http:// www.interscience.wiley.com/jpages/1045-2257/suppmat. (c) 2006 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ATAXIA-TELANGIECTASIA HETEROZYGOTES; SEQUENCE VARIANTS; PROTEIN FUNCTION; DNA-DAMAGE; RISK; MISSENSE; CARRIERS; FAMILIES; BRCA1; SUSCEPTIBILITY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Jan 2021 07:26 |
| Last Modified: | 18 Jan 2021 07:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33685 |
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