Suzuki, Toru and Asami, Maki and Hoffmann, Martin and Lu, Xin and Guzvic, Miodrag and Klein, Christoph A. and Perry, Anthony C. F. (2016) Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes. NATURE COMMUNICATIONS, 7: 12676. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full-term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodelling of histones and genomic 5'-methylcytosine and 5'-hydroxymethylcytosine following embryo injection were distinct from remodelling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MOUSE ZYGOTES; MEIOTIC EXIT; MAMMALIAN ZYGOTE; CHROMATIN; EMBRYOS; OOCYTES; CELLS; EXPRESSION; GENE; FERTILIZATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für experimentelle Medizin und Therapieverfahren |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Apr 2019 11:27 |
| Last Modified: | 04 Apr 2019 11:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3374 |
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